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human dmd myoblasts  (PromoCell)


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    PromoCell human dmd myoblasts
    (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or <t>untreated</t> <t>myoblasts.</t> (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D <t>DMD</t> muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.
    Human Dmd Myoblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dmd myoblasts/product/PromoCell
    Average 98 stars, based on 472 article reviews
    human dmd myoblasts - by Bioz Stars, 2026-03
    98/100 stars

    Images

    1) Product Images from "CRISPR-Cas9 mediated endogenous utrophin upregulation improves Duchenne Muscular Dystrophy"

    Article Title: CRISPR-Cas9 mediated endogenous utrophin upregulation improves Duchenne Muscular Dystrophy

    Journal: bioRxiv

    doi: 10.1101/2023.04.18.536394

    (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or untreated myoblasts. (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D DMD muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.
    Figure Legend Snippet: (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or untreated myoblasts. (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D DMD muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.

    Techniques Used: Generated, Real-time Polymerase Chain Reaction, Expressing, Muscles, Western Blot, Immunofluorescence, Staining, Control, Immunostaining



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    human dmd myoblasts  (PromoCell)
    98
    PromoCell human dmd myoblasts
    (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or <t>untreated</t> <t>myoblasts.</t> (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D <t>DMD</t> muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.
    Human Dmd Myoblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dmd myoblasts/product/PromoCell
    Average 98 stars, based on 1 article reviews
    human dmd myoblasts - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or untreated myoblasts. (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D DMD muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.

    Journal: bioRxiv

    Article Title: CRISPR-Cas9 mediated endogenous utrophin upregulation improves Duchenne Muscular Dystrophy

    doi: 10.1101/2023.04.18.536394

    Figure Lengend Snippet: (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or untreated myoblasts. (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D DMD muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.

    Article Snippet: Human DMD myoblasts were maintained in Smooth Muscle Cell Growth Medium (C-23060, PromoCell) supplemented with 20% fetal bovine serum gold (PAA) and 1% penicillin– streptomycin (Invitrogen).

    Techniques: Generated, Real-time Polymerase Chain Reaction, Expressing, Muscles, Western Blot, Immunofluorescence, Staining, Control, Immunostaining